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Toluidine Blue O 1%
w/v with 1% v/v Acetic Acid in 20% v/v Denatured Ethanol
| Item # | T1009 |
|---|---|
| Weight | N/A |
| Dimensions | N/A |
| Available Size(s) | 60mL, 120mL |
| Manufacturer | |
| Packaging Type | Poly w/ Fliptop |
| Sample COA | View/Download Sample COA |
Ready-to-use reagent. Prepared with ACS reagent grade Toluidine Blue, Acetic Acid and Reagent Alcohol. Suitable for laboratory use.
Product Overview
Toluidine Blue O is a basic thiazine dye used extensively in histology and cytology for its metachromatic staining properties. This formulation is prepared in a stabilized matrix of 1% v/v acetic acid and 20% v/v denatured ethanol, ensuring reagent clarity, enhanced shelf life, and optimal staining performance.
The dye binds strongly to acidic tissue components such as nucleic acids, mucopolysaccharides, and mast cell granules. It displays a characteristic color shift—blue in monomeric form, and purple or red in the presence of highly acidic or polymerized substrates—making it ideal for distinguishing tissue architecture and cellular details.
Key Features:
- Metachromatic staining in a stabilized ethanol-acid matrix
- Targets nuclei, cartilage, mast cells, and mucins
- High tissue contrast and rapid staining performance
- Ideal for paraffin, resin, or smear preparations
- Long shelf life with consistent results
Applications:
- Histological examination of connective tissue and epithelial structures
- Detection of mast cells and acidic polysaccharides in diagnostic samples
- Screening of oral and cervical smears
- Semi-thin resin section staining in EM workflows
- Educational settings for demonstrating cell and tissue morphology
Toluidine Blue O in this stabilized formulation offers reliable, vivid metachromatic staining for both routine diagnostic use and advanced tissue analysis.
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