Product Overview

Our Acetate Buffer, pH 4.00 is a high-quality, ready-to-use acidic buffer system formulated for applications requiring tight pH control in the acidic range. Composed of acetic acid and sodium acetate, this buffer maintains a stable pH of 4.00 at 25 °C, making it ideal for a variety of biochemical assays, titrations, extraction protocols, and instrument calibration tasks.

Widely used in enzyme activity studies, pharmaceutical analysis, and protein purification workflows, acetate buffer provides effective buffering capacity in the pH 3.6–5.6 range, with pH 4.0 as a standard reference point for acidic reaction environments.

Key Features:

• Maintains pH 4.00 ± 0.05 at 25 °C
• Prepared using high-purity acetic acid and sodium acetate
• Excellent for acidic protein chemistry, analytical methods, and buffer exchange
• Available in convenient volumes (e.g., 500 mL, 1 L, 3.8 L)
• Colorless, ready-to-use, and stable for extended storage

Applications:

• pH control in enzyme assays (e.g., pepsin, β-galactosidase)
• Sample preparation and buffer exchange in chromatography
• Pharmaceutical QC and pKa determination
• Useful as a reference or supporting buffer alongside pH calibration buffers (pH 4.01, 7.00, 10.01)

Acetate Buffer pH 4.00 provides reliable acidic buffering capacity for a wide range of laboratory, clinical, and industrial workflows, offering stable performance and trusted reproducibility.