Product Overview

Alizarin Red S is a highly sensitive azo dye indicator commonly used for the colorimetric detection of calcium and other divalent metal ions in aqueous solutions and biological specimens. In analytical chemistry, it serves as a complexometric indicator, forming a distinct red to orange complex with calcium (Ca²⁺), magnesium (Mg²⁺), barium (Ba²⁺), and other metals—providing visual confirmation in titrations and chelation studies.

In biological research, Alizarin Red S is widely used for histological staining of calcified tissues, particularly in bone and cartilage, to detect calcium deposition in cell cultures, embryos, and histological sections. It binds to calcium salts and produces a vivid orange-red coloration, allowing easy visualization under light microscopy.

Key Features:

• Used as a complexometric indicator for detecting Ca²⁺, Mg²⁺, Ba²⁺, and similar ions
• Produces an orange-red complex upon metal ion binding
• Commonly used in EDTA titrations for calcium quantification
• Ideal for osteogenesis assays and bone mineralization studies
• Available in powder or ready-to-use aqueous solution

Applications:

• Analytical Chemistry: Metal ion detection and EDTA complexometric titrations
• Histology: Calcium staining in fixed tissues (e.g., bone, teeth, cartilage)
• Cell Biology: Visualization of extracellular matrix calcification in in vitro studies
• Tissue Engineering: Evaluation of osteogenic differentiation in stem cell cultures

Alizarin Red S Indicator is a trusted reagent for researchers and analysts in clinical diagnostics, environmental testing, and biomedical sciences, offering visual sensitivity, reliability, and versatility across both chemical and biological workflows.