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Biuret Reagent

Item # B1033
Weight N/A
Dimensions N/A
Available Size(s)

1L, 120mL, 500mL, 3.8L

Manufacturer

Packaging Type

Poly, Poly Gallon, Poly w/ Fliptop

Safety Data Sheet View/Download SDS
Sample COA View/Download Sample COA

Protein Detection Solution for Colorimetric Analysis

Product Overview

Biuret Reagent is a colorimetric assay solution used to detect the presence of proteins and peptides in a sample by reacting with peptide bonds. When proteins are present, the reagent produces a distinct violet or purple color due to the formation of a complex between copper ions and peptide linkages in an alkaline environment.

Widely used in biochemistry, clinical labs, food testing, and educational demonstrations, the Biuret test is a quick and reliable method for qualitative and semi-quantitative analysis of protein concentration. The intensity of the color correlates to the amount of protein present, making it suitable for comparative testing and instructional applications.


Key Features:

  • Detects proteins and peptides by targeting peptide bonds
  • Produces a violet to purple color in the presence of protein
  • Used in clinical diagnostics, nutrition testing, and biology education
  • Simple and effective for semi-quantitative analysis
  • Requires alkaline conditions for optimal performance
  • Provides clear, visible results within minutes

Color Response:

  • Blue – No protein present
  • Purple/Violet – Presence of proteins or long peptide chains
  • Pinkish – Presence of smaller peptides or low concentrations

Biuret Reagent is a fundamental tool for protein detection and quantification, valued for its ease of use, accuracy, and versatility in both laboratory and instructional environments.

 

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