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Methyl Orange-Xylene Cyanol Indicator

w/v Aqueous
Item # M1013
Weight N/A
Dimensions N/A
Available Size(s)

1L, 120mL, 500mL, 3.8L

Manufacturer

Packaging Type

Poly, Poly Gallon, Poly w/ Fliptop

Safety Data Sheet View/Download SDS
Sample COA View/Download Sample COA

pH 2.9 (purple) - pH 4.6 (green) Ready-to-use reagent. Prepared with ACS reagent grade Methyl Orange, Xylene Cyanol and high purity water. Suitable for laboratory use.

Product Overview

Methyl Orange–Xylene Cyanol is a specialized dye mixture that combines the pH sensitivity of methyl orange with the tracking properties of xylene cyanol. This blend is used for visualizing pH transitions in acidic environments while also serving as a migration marker in electrophoresis and other dye-front tracking applications.

Methyl orange provides a sharp red-to-yellow color shift between pH 3.1 and 4.4, while xylene cyanol migrates as a slow-moving, blue-colored tracking dye in nucleic acid gel electrophoresis. Together, they offer dual functionality in both analytical and molecular workflows.

Key Features:

  • pH transition range from 3.1 to 4.4 (methyl orange)
  • Xylene cyanol offers a blue migration marker in gel systems
  • Useful in combined pH detection and electrophoresis loading dye formats
  • Available in dry blend or aqueous solution
  • Stable under normal storage and handling conditions

Applications:

  • Acid-base titrations and pH monitoring
  • Electrophoresis dye for tracking DNA/RNA sample migration
  • Indicator in teaching demonstrations involving dye separation
  • Colorimetric tests involving dual-dye performance
  • Analytical workflows needing both pH indication and visual tracking

Methyl Orange–Xylene Cyanol provides the versatility of a dual-purpose indicator and tracking dye, making it a valuable tool for laboratories conducting both analytical chemistry and molecular biology procedures.

 

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